Standard Mouse Monoclonal Antibody Production Service

Standard Mouse Mab Production Service Introduction

Mouse mAb has many features such as targeting to a single epitope, high specificity, stable passage, large-scale repeated production and extensive applications in basic research, medical diagnosis, tumor therapy and radioimmunoimaging. Mouse mAb developed by Sino Biological has the advantages of high sensitivity & specificity. We can also provide flexible customized solutions according to customer requirements, aiming to provide customers with high-quality antibodies.

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Standard Mouse Mab Production Service Process

Service procedures Specification Timeline Deliverables Guarantee Price

Antigen preparationQuote!
Please refer to antigen production service Please fill in the form and send it to
[email protected]

Antigen validation
? Analysis of client's antigen by SDS-PAGE and UV 1-2 days ? 2-5 strains of ELISA positive clones
(2 tubes of cryopreserved cells)
? 2-5mL supernatant per clone
? 1-3mg purified antibody (choose a hybridoma cell)
? Experimental report
ELISA positive for immunogen

Immunization and serum titer test
? Pre-immune bleed
? Five Balb/c mice immunization
? Serum titer test
? Final bleed
8-10 weeks

Fusion and Screening
? Fusion and Screening
? Subcloning and cell expansion
? Cell Banking
4-6 weeks

Antibody production & purification
? Appropriate scale hybridoma cell culture
? Purified by protein A affinity chromatography
2-3 weeks

QC analysis
? Analysis by SDS-PAGE and UV
? ELISA validation
3 days
User-supplied antigen requirements:
Recombinant protein antigen Peptide antigen
Antigen quantities 3-4mg/5 mice ? KLH/VLP conjugated peptides 5mg/5 mice
Antigen Size? >10kD ? Concentration? >0.5mg/mL?
SDS-Page purity? >90% ? OVA/Biotin conjugated peptides? 2-3mg
Concentration? >0.5mg/mL?   Concentration? >0.1mg/mL?
Formulation? PBS, if not PBS, please inquire first   Formulation PBS, if not PBS, please inquire first

Case Presentation of Standard Mouse Mab Production Service

Sino Biological Inc. has established a high-throughput mouse monoclonal antibody R&D platform. A set of high-throughput R&D programs for mouse monoclonal antibodies are also established through the optimization of immunization protocols, fusion protocols, screening protocols, and high-throughput antibody production and purification protocols. All the mouse Mab are validated by ELISA, WB, IHC, FACS et al. , and are tested by multi-tissue and multi-cell.

a)Multi-cell, multi-tissue validation

  • ? Human placenta
    Fig 1. Immunochemical staining of human target D with mouse Mab. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen.
  • ? Human breast carcinoma
    Fig 2. Immunochemical staining of human target D with mouse monoclonal antibody.

b)Multi-application verification

  • Fig 1. Confocal immunofluorescence analysis of human target D in MCF7 cells. Positive staining was localized to lysosome membrane.
  • Fig 2. Flow cytometric analysis of human target D on Jurkat cells. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
  • Fig 3. Lane A: Jurkat Whole Cell Lysate. Anti-target D mouse Mab; goat Anti-mouse IgG H&L (Dylight800).
  • Fig 4. Lane A: Hela Whole Cell Lysate; Lane B: Jurkat Whole Cell Lysate; Lane C: Daudi Whole Cell Lysate. Anti-target D mouse Mab; Dylight 800-labeled antibody to Mouse IgG (H+L).

Standard Mouse Mab Production Service Advantages

  • International leading recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience on 6000+ protein production, high success rate of antigen expression.
  • Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95%.
  • Customized immunization, screening and detection proposed according to customer's final application requirements can be provided.
  • ELISA, WB, IHC, FACS and other validation platforms, multi-cell and multi-tissue verification, aiming to improve antibody screening success rate with low background and high sensitivity.
  • Quoting by steps, charging by nodes, reducing customer's risk and saving costs.
  • Optimized electrofusion technology has more than 3-5 times efficiency compared with PEG mediated cell fusion.

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