Sino Biological Inc. provides scientists worldwide with multiple forms of cDNA clones including expression-ready cDNA ORF clones, lentivirus cDNA ORF clones, and cDNA Clone in cloning vectors. All gene products were verified by full-length sequencing to ensure high-quality cDNA clones. Sino Biological has always been committed to providing customers with high quality products and services.
Advantages of cDNA Clones
- Gene Bank: 11,000+ molecules, 400,000+ cDNA clones, most are ready to ship
- Multiple Species: Human、Mouse、Rat、Rabbit、Rhesus、Cynomolgus、Canine、Ferret、zebra fish、Virus...
- Multiple Tags: N-terminal or C-terminal Myc、HA、FLAG、His、GFPSpark? and OFPSpark?
- Influenza Virus Gene Bank: 60 subtypes, 160 strains, including H7N9, H5N1 and other popular strains; and genes of nearly 40 kinds of viruses such as MERS COV, HCV, RSV, HCov...
- Full length sequence confirmed: Each gene product is verified by full-length sequencing; ~100% sequence accuracy
- Customized service: Meet customer's needs
Types of cDNA Clone Products
| Sequence Verification | Full length sequence confirmed |
| Expression Validated | Yes |
| Expression Host | Mammalian cell |
| Vectors | pCMV/pCMV3-Series |
| Tags | untagged、N-/C-Flag/HA/His/Myc/GFPSpark?/OFPSpark? |
| Application | Protein overexpression; protein detection and purification; cell localization; stable strain construction |
| Features | Expression validated, quality assurance |
| Sequence Verification | Full length sequence confirmed |
| Expression Validated | Yes |
| Expression Host | Mammalian cell |
| Vectors | pLV-Series |
| Tags | untagged、N-/C-Flag/HA/His/Myc/GFPSpark?/OFPSpark? |
| Application | Packaging lentivirus; protein overexpression; stable strain construction |
| Features | Transfection of dividing and non-dividing cells, primary cells, stem cells and other difficult-to-transfect cells |
Expression-ready ORF Clone: Expression Validated
-
Human PTPN1, N-Flag
(HG10304-NF) -
Human IDH1, N-GFPSpark?
(HG12055-ANG) -
Human SIRT6, C-GFPSpark?
(HG12156-ACG) -
H7N9-NP, C-Flag
(VG40110-CF) -
Human PKM2, C-OFPSpark?
(HG11430-ACR) -
Human MTOR, C-OFPSpark?
(HG11508-ACR)
Featured cDNA Clones：Organelle Marker Plasmid
Sino Biological provides a collection of fluorescent fusion proteins that are targeted to various organelles or subcellular structures. The fluorescent fusion protein is introduced using a simple transfection step, after 48h, you can take the beautiful images directly by fluorescence microscopy.
| GFPSpark? or OFPSpark? fluorescent colors for choice | Easy labeling of cell compartments and proteins |
| Perfect for study of localization and trafficking | No antibody or chemical treatment required |
cDNA Clone Quality Control Standards
cDNA clones are controlled by strict quality control and Sino Biological only provides you with high quality plasmids:
a) One plasmid was sequenced twice to ensure sequence accuracy
b) UV and electrophoresis：A260/A280 = 1.8~2.0, guarantee plasmid purity
c) PCR identification：Identify gene size and exclude plasmid contamination
cDNA Clone Product Citations(Partial)
| Organization | Molecule | Catalog No. | Reference Citations | Published Journals |
| Georgia Regents University, etc. | ISL1 | HG13122-G | DNMT1 is essential for mammary and cancer stem cell maintenance and tumorigenesis | Nat Commun |
| Swiss Federal Institute of Technology |
IL9 | MG51302-G | Glycosylation profiles determine extravasation and disease-targeting properties of armed antibodies | Proc. Natl. Acad. Sci. U.S.A. |
| Cincinnati Children's Hospital Medical Center,etc. | SMAD3 | MG50991-UT | TGFβ signaling inhibits goblet cell differentiation via SPDEF in conjunctival epithelium | Development |
| INRS-Institut-Armand-Frappier | BMP-4 | HG10609-CF | Bone morphogenetic protein 4 (BMP-4) and epidermal growth factor (EGF) inhibit metalloproteinase-9 (MMP-9) expression in cancer cells | Oncoscience |
| Jinling Hospital, School of Medicine, Nanjing University | GSK3b | HG10044-M | MicroRNA-451 induces epithelial–mesenchymal transition in docetaxel-resistant lung adenocarcinoma cells by targeting proto-oncogene c-Myc | European Journal of Cancer |
| The Johns Hopkins University,etc. | furin | HG10141-M | Preparation and properties of a papillomavirus infectious intermediate and its utility for neutralization studies | Virology |
cDNA Clone Related Products
Molecular Biology Service
- Molecular Biology Service
- Real Time PCR Service
- ? Real Time PCR Primer and Probe Design
- ? Real Time Quantitative PCR Analysis
