Application | Dilution |
---|---|
WB | 1:500-1:2000 |
IHC-P | 1:500-1:2000 |
ICC-IF | 1:300-1:10000 |
IP | 4-8 μL/mg of lysate |
CALB1 was immunoprecipitated using:
Lane A:0.5 mg HepG2 Whole Cell Lysate
Lane B:0.5 mg 293T Whole Cell Lysate
4 μL anti-CALB1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.Primary antibody:Anti-CALB1 rabbit polyclonal antibody,at 1:100 dilution
Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution
Developed using the odssey technique.Performed under reducing conditions.
Predicted band size: 30 kDaObserved band size: 30 kDa
Anti-CALB1 rabbit polyclonal antibody at 1:500 dilution.
Lane A: Mosue kidney tissue lysate
Lysates/proteins at 30 μg per lane.Secondary
Goat Anti- Rabbit??IgG H&L (Dylight 800)??at 1/10000 dilution.
Developed using the Odyssey technique.Performed under reducing conditions.
Predicted band size:30 kDaObserved band size:27 kDa
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