Anti-Calbindin Antibody
Cat: 102051-T46
Anti-Calbindin Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Calbindin Antibody
Validated applications
WB,IHC-P,ICC/IF,IP
Species reactivity
Reacts with: Human
Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
Specificity
Human Calbindin
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human Calbindin
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Calbindin, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Anti-Calbindin Antibody (Rabbit Polyclonal antibody) Validated Applications
| Application | Dilution |
|---|---|
| WB | 1:500-1:2000 |
| IHC-P | 1:500-1:2000 |
| ICC-IF | 1:300-1:10000 |
| IP | 4-8 μL/mg of lysate |
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Anti-Calbindin Antibody (Rabbit Polyclonal antibody) Images
-HD09AU1826-1ug-293-IF.png)
Immunofluorescence staining of CALB1 in 293 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human CALB1 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor?488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.
-HD09AU1826-1ug-brain-IHC-P.png)
Immunochemical staining of human CALB1 in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
-HD09AU1826-IP.png)
CALB1 was immunoprecipitated using:
Lane A:0.5 mg HepG2 Whole Cell Lysate
Lane B:0.5 mg 293T Whole Cell Lysate
4 μL anti-CALB1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.Primary antibody:Anti-CALB1 rabbit polyclonal antibody,at 1:100 dilution
Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution
Developed using the odssey technique.Performed under reducing conditions.
Predicted band size: 30 kDaObserved band size: 30 kDa
-HD09AU1826-WB.png)
Anti-CALB1 rabbit polyclonal antibody at 1:500 dilution.
Lane A: Mosue kidney tissue lysate
Lysates/proteins at 30 μg per lane.Secondary
Goat Anti- Rabbit??IgG H&L (Dylight 800)??at 1/10000 dilution.
Developed using the Odyssey technique.Performed under reducing conditions.
Predicted band size:30 kDaObserved band size:27 kDa
Anti-Calbindin Antibody: Alternative Names
Anti-CALB Antibody; Anti-D-28K Antibody
Calbindin Background Information
Full Name
calbindin 1, 28kDa
Recombinant Antibody Production Service
High-throughput Recombinant Antibody Production
